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KMID : 0359320080480010033
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Korean Journal of Veterinary Research
2008 Volume.48 No. 1 p.33 ~ p.38
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Determination of amitraz by high-performance Liquid chromatography with photodiode array detection and method validation
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Yun Hyeong-Jun
Yun So-Mi
Lee Myung-Heon
Son Seong-Wan
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Abstract
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In veterinary medicine amitraz has been used as an insecticide to eliminates mites, lice, and ticks in dogs, cats, goats, swine and cattle. The objective of present study was to developed an analytical method using one-step extraction and determination of the amitraz in veterinary drugs by liquid chromatography (LC). The amitraz was analyzed by LC equipped with Waters XTerra RP18 (4.8 × 250 mm; 5 μm; Waters, USA) analytical column, using 75% acetonitrile (acetonitrile/D.W; 75/25) at 1.0 ml/ min. The UV-VIS detection of amitraz was made at 290 nm. Calibration graphs were linear with very good correlation coefficients (r2 > 0.9999) from 80~120 μg/ml. The limit of detection was 0.09 μg/ml and
limit of quantification was 0.27 μg/ml. The method showed good intra-day precision (CV 0.05~0.09%) and inter-day precision (CV 0.06~0.18%).
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KEYWORD
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amitraz, LC, veterinary drug
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